The enzymatic removal of a surfactant coating from quartz and kaolin by P388D1 cells
Identifieur interne : 002B35 ( Main/Exploration ); précédent : 002B34; suivant : 002B36The enzymatic removal of a surfactant coating from quartz and kaolin by P388D1 cells
Auteurs : C. A. Hill [États-Unis] ; W. E. Wallace [États-Unis] ; M. J. Keane [États-Unis] ; P. S. Mike [États-Unis]Source :
- Cell Biology and Toxicology [ 0742-2091 ] ; 1995-04-01.
English descriptors
- KwdEn :
- Teeft :
- Academic press, Alveolar macrophages, American type culture collection, Cell death, Cell line, Cell number, Culture medium, Digestion, Digestion experiments, Digestion studies, Double bonds, Dust concentrations, Dust level, Dust particles, Dust surface, Enzymatic removal, Error bars, Extracellular, Extracellular phospholipase, Extracellular phospholipase activity, Glass wool plug, Highest concentration, Inner layer, Kaolin, Kaolin dust, Lecithin surfactant, Lipid, Macrophage, Mineral dusts, Mineral surface, Occupational safety, Organic phase, Osmium tetroxide, Palmitic, Palmitic acid, Palmitic acid chains, Phospholipase, Phospholipase activity, Protective coating, Pulmonary surfactant, Quartz, Respirable quartz, Room temperature, Rpmi, Rubber policeman, Specific surface area, Standard deviation, Statistical analysis, Stock solution, Surfactant, Test tube, Total cell counts, Total lipids, Unlabeled quartz, Viability.
Abstract
Abstract: The macrophage-like cell line, P388D1, was exposed to dipalmitoyl lecithin (DPL)-coated respirable quartz and kaolin, and the disappearance of the DPL was monitored for up to 9 days. The coating was removed rapidly at first (about 50% in the first 3 days) and then more slowly over the remaining 6 days, until about 30% remained on day 9. The rate of DPL digestion was independent of the type of dust and the amount of coated dust within the cell, indicating the existence of an extracellular phospholipase activity. This extracellular phospholipase activity was partially characterized. It was sensitive to temperatures above 56°C, the presence of EDTA, the action of the proteases trypsin and proteinase K, and pH, being active at pH 7 but not at pH 5. This is consistent with reports in the leterature of the existence of an extralysosomal phospholipase which is active at pH 7 and dependent on the presence of divalent metal ions. There was a dust-dependent difference in the extracellular rate of DPL digestion from quartz and kaolin. The coating was removed more slowly from the kaolin than it was from quartz. The removal of the DPL coating seen in the presence of cells was presumably due to both an intracellular and an extracellular phospholipase.
Url:
DOI: 10.1007/BF00767497
Affiliations:
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Mike</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:575FFB8CB776BF2E8E927050BACCF007411D8428</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF00767497</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-2JJTQ4VC-0/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000E74</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E74</idno><idno type="wicri:Area/Istex/Curation">000E74</idno><idno type="wicri:Area/Istex/Checkpoint">001926</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001926</idno><idno type="wicri:doubleKey">0742-2091:1995:Hill C:the:enzymatic:removal</idno><idno type="wicri:Area/Main/Merge">002B93</idno><idno type="wicri:Area/Main/Curation">002B35</idno><idno type="wicri:Area/Main/Exploration">002B35</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The enzymatic removal of a surfactant coating from quartz and kaolin by P388D1 cells</title><author><name sortKey="Hill, C A" sort="Hill, C A" uniqKey="Hill C" first="C. A." last="Hill">C. A. Hill</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Respiratory Disease Research, National Institute of Occupational Safety and Health, 1095 Willowdale Road, 26505-2845, Morgantown, West Virginia</wicri:regionArea><placeName><region type="state">Virginie-Occidentale</region></placeName></affiliation></author><author><name sortKey="Wallace, W E" sort="Wallace, W E" uniqKey="Wallace W" first="W. E." last="Wallace">W. E. Wallace</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Respiratory Disease Research, National Institute of Occupational Safety and Health, 1095 Willowdale Road, 26505-2845, Morgantown, West Virginia</wicri:regionArea><placeName><region type="state">Virginie-Occidentale</region></placeName></affiliation></author><author><name sortKey="Keane, M J" sort="Keane, M J" uniqKey="Keane M" first="M. J." last="Keane">M. J. 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Mike</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Respiratory Disease Research, National Institute of Occupational Safety and Health, 1095 Willowdale Road, 26505-2845, Morgantown, West Virginia</wicri:regionArea><placeName><region type="state">Virginie-Occidentale</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Biology and Toxicology</title><title level="j" type="abbrev">Cell Biol Toxicol</title><idno type="ISSN">0742-2091</idno><idno type="eISSN">1573-6822</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1995-04-01">1995-04-01</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="119">119</biblScope><biblScope unit="page" to="128">128</biblScope></imprint><idno type="ISSN">0742-2091</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0742-2091</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>P388D1</term><term>dipalmitoyl phosphatidyl choline</term><term>kaolin</term><term>quartz</term><term>surfactant</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Alveolar macrophages</term><term>American type culture collection</term><term>Cell death</term><term>Cell line</term><term>Cell number</term><term>Culture medium</term><term>Digestion</term><term>Digestion experiments</term><term>Digestion studies</term><term>Double bonds</term><term>Dust concentrations</term><term>Dust level</term><term>Dust particles</term><term>Dust surface</term><term>Enzymatic removal</term><term>Error bars</term><term>Extracellular</term><term>Extracellular phospholipase</term><term>Extracellular phospholipase activity</term><term>Glass wool plug</term><term>Highest concentration</term><term>Inner layer</term><term>Kaolin</term><term>Kaolin dust</term><term>Lecithin surfactant</term><term>Lipid</term><term>Macrophage</term><term>Mineral dusts</term><term>Mineral surface</term><term>Occupational safety</term><term>Organic phase</term><term>Osmium tetroxide</term><term>Palmitic</term><term>Palmitic acid</term><term>Palmitic acid chains</term><term>Phospholipase</term><term>Phospholipase activity</term><term>Protective coating</term><term>Pulmonary surfactant</term><term>Quartz</term><term>Respirable quartz</term><term>Room temperature</term><term>Rpmi</term><term>Rubber policeman</term><term>Specific surface area</term><term>Standard deviation</term><term>Statistical analysis</term><term>Stock solution</term><term>Surfactant</term><term>Test tube</term><term>Total cell counts</term><term>Total lipids</term><term>Unlabeled quartz</term><term>Viability</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The macrophage-like cell line, P388D1, was exposed to dipalmitoyl lecithin (DPL)-coated respirable quartz and kaolin, and the disappearance of the DPL was monitored for up to 9 days. The coating was removed rapidly at first (about 50% in the first 3 days) and then more slowly over the remaining 6 days, until about 30% remained on day 9. The rate of DPL digestion was independent of the type of dust and the amount of coated dust within the cell, indicating the existence of an extracellular phospholipase activity. This extracellular phospholipase activity was partially characterized. It was sensitive to temperatures above 56°C, the presence of EDTA, the action of the proteases trypsin and proteinase K, and pH, being active at pH 7 but not at pH 5. This is consistent with reports in the leterature of the existence of an extralysosomal phospholipase which is active at pH 7 and dependent on the presence of divalent metal ions. There was a dust-dependent difference in the extracellular rate of DPL digestion from quartz and kaolin. The coating was removed more slowly from the kaolin than it was from quartz. The removal of the DPL coating seen in the presence of cells was presumably due to both an intracellular and an extracellular phospholipase.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Virginie-Occidentale</li></region></list><tree><country name="États-Unis"><region name="Virginie-Occidentale"><name sortKey="Hill, C A" sort="Hill, C A" uniqKey="Hill C" first="C. A." last="Hill">C. A. Hill</name></region><name sortKey="Keane, M J" sort="Keane, M J" uniqKey="Keane M" first="M. J." last="Keane">M. J. Keane</name><name sortKey="Mike, P S" sort="Mike, P S" uniqKey="Mike P" first="P. S." last="Mike">P. S. Mike</name><name sortKey="Wallace, W E" sort="Wallace, W E" uniqKey="Wallace W" first="W. E." last="Wallace">W. E. Wallace</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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